Plasmid_Backbone

Part:BBa_J176126:Design

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-10-23)


V0201


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1953
    Illegal XbaI site found at 1685
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1953
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2043
    Illegal NotI site found at 1024
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1953
    Illegal BglII site found at 361
    Illegal BglII site found at 707
    Illegal BamHI site found at 104
    Illegal BamHI site found at 450
    Illegal XhoI site found at 73
    Illegal XhoI site found at 392
    Illegal XhoI site found at 419
    Illegal XhoI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1953
    Illegal XbaI site found at 1685
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1953
    Illegal XbaI site found at 1685
    Illegal SpeI site found at 1044
    Illegal PstI site found at 2043
    Illegal NgoMIV site found at 241
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 1546
    Illegal NgoMIV site found at 2808
    Illegal AgeI site found at 1111
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1093
    Illegal BsaI.rc site found at 4336
    Illegal SapI site found at 3253


Design Notes

TBA


Source

TBA

References